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Clostridium difficile [Meth...
This second edition provides 21 new chapters on methods used in laboratories for investigating the physiology and molecular genetics of the pathogen Clostridium difficile. Chapters detail up-to -date experimental techniques for gene editing and transcriptional analysis which are used to investigate the fundamental biology of the organism and its virulence factors. Additional chapters describe development of potential new treatments including vaccines, bacteriophage and faecal transplantation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Clostridium difficile: Methods and Protocols, Second Edition provides a comprehensive catalogue of molecular tools and techniques authored by the researchers who have developed them
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monografia Rebiun18082141 https://catalogo.rebiun.org/rebiun/record/Rebiun18082141 cr nn 008mamaa 160902s2016 xxu| s |||| 0|eng d 9781493963614 10.1007/978-1-4939-6361-4 doi UEM 393654 CBUC 991000634459706708 CBUC 991034167799706706 UPVA 996874449703706 CBUC 991010403887006709 CBUC 991010403887006709 MJCM bicssc MED044000 bisacsh 616.079 23 Clostridium difficile Recurso electrónico-En línea] Methods and Protocols edited by Adam P. Roberts, Peter Mullany 2nd ed. 2016 New York, NY Springer New York Imprint: Humana Press 2016 New York, NY New York, NY Springer New York Imprint: Humana Press XI, 292 p. 49 illus., 33 illus. in color. online resource XI, 292 p. 49 illus., 33 illus. in color. Text txt rdacontent computer c rdamedia online resource cr rdacarrier Methods in Molecular Biology 1064-3745 1476 Springer Protocols (Springer-12345) Restriction Endonuclease Analysis Typing of Clostridium Difficile Isolates -- Direct PCR-ribotyping of Clostridium difficile -- From FASTQ to Function: In silico Methods for Processing Next Generation Sequencing Data -- Clostridium difficile Genome Editing Using pyrE Alleles -- Use of mCherryOpt Fluorescent Protein in Clostridium difficile -- A Fluorescent Reporter for Single Cell Analysis of Gene Expression in Clostridium difficile -- Clostridium difficile Adhesins -- Intestinal Epithelial Cell Response to Clostridium difficile Flagella -- Isolating and Purifying Clostridium difficile Spores -- Inducing and Quantifying Clostridium difficile Spore Formation -- Characterization of Functional Prophages in Clostridium difficile -- Induction and Purification of C. difficile Phage Tail-like Particles -- Phage Transduction -- Transfer of Clostridium difficile Genetic Elements Conferring Resistance to Macrolide-Lincosamide-streptograminB (MLSB) Antibiotics -- Methods for Determining Transfer of Mobile Genetic Elements in Clostridium difficile -- Investigating Transfer of Large Chromosomal Regions Containing the Pathogenicity Locus between Clostridium difficile Strains -- An in vitro Model of the Human Colon: Studies of Intestinal Biofilms and lostridium difficile Infection -- MiniBioReactor Arrays (MBRAs) as a Tool for Studying C. difficile Physiology in the Presence of a Complex Community -- A Practical Method for Preparation of Fecal Microbiota Transplantation -- Ion Exchange Chromatography to Analyze Components of a Clostridium difficile Vaccine -- A Size Exclusion Chromatography Method for Analysis of Clostridium difficile Vaccine Toxins Accesible sólo para usuarios de la UPV Recurso a texto completo This second edition provides 21 new chapters on methods used in laboratories for investigating the physiology and molecular genetics of the pathogen Clostridium difficile. Chapters detail up-to -date experimental techniques for gene editing and transcriptional analysis which are used to investigate the fundamental biology of the organism and its virulence factors. Additional chapters describe development of potential new treatments including vaccines, bacteriophage and faecal transplantation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Clostridium difficile: Methods and Protocols, Second Edition provides a comprehensive catalogue of molecular tools and techniques authored by the researchers who have developed them Reproducción electrónica Forma de acceso: Web Medicine Immunology Biomedicine Immunology Roberts, Adam P. editor Mullany, Peter. editor SpringerLink (Servicio en línea) Springer eBooks Springer eBooks Printed edition 9781493963591 Methods in Molecular Biology 1064-3745 1476