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Adhesion Protein Protocols ...
Cellular adhesion is a fundamental process that influences numerous biological activities such as morphogenesis, cell motility and division, as well as signalling. In addition, adhesion is a process important not only in normal physiology and development, but also in disease states such as tumourigenesis, cardiovascular disease, inflammation and infection. There are a plethora of proteins involved in adhesion-related events with a huge diversity in function. As a result, a wide variety of techniques exist to study adhesion related proteins and processes. In Adhesion Protein Protocols, Third Edition, chapters cover techniques to gain insight into the complex and incompletely understood processes that are involved in cellular adhesion. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Adhesion Protein Protocols, Third Edition will be useful for both those new to the field of adhesion protein research as well as the more experienced scientist
Monografía
monografia Rebiun02166513 https://catalogo.rebiun.org/rebiun/record/Rebiun02166513 |acr nuu---uuuuu 130718s2013 xxu| s |||| 0|eng d 9781627035378 9781627035385 10.1007/978-1-62703-538-5 doi UEM 392760 UPVA 998394473903706 CBUC 991010835294806709 CBUC 991003332419706706 CBUC 991009364929706708 BUS 572.6 23 Adhesion Protein Protocols Recurso electrónico] edited by Amanda S. Coutts 3rd ed. 2013 Servicio en línea Totowa, NJ Humana Press Imprint: Humana Press 2013 Totowa, NJ Totowa, NJ Humana Press Imprint: Humana Press XII, 407 p. 87 illus., 57 illus. in color. online resource XII, 407 p. 87 illus., 57 illus. in color. Methods in Molecular Biology, Methods and Protocols 1064-3745 1046 Libros electrónicos descargables Reconstructing Integrin Activation in vitro -- Quantifying Cellular Adhesion to Covalently Immobilized Extracellular Matrix Proteins by Single-Force Spectroscopy -- A Multimode-TIRFM & Microfluidic Technique to Examine Platelet Adhesion Dynamics -- Localization-Based Super-Resolution Imaging of Cellular Structures -- Use of Microarray Analysis to Investigate EMT Gene Signatures.- Podosome Reformation in Macrophages: Assays and Analysis -- Mobile and Three-Dimensional Presentation of Adhesion Proteins Within Microwells -- Basement Membrane Invasion Assays: Native Basement Membrane and Chemoinvasion Assay -- Cytoplasmic Actin: Purification and Single Molecule Assembly Assays -- Synthetic and Tissue-Derived Models for Studying Rigidity Effects on Invadopodia Activity -- Determining Rho GTPase Activity by an Affinity-Precipitation Assay -- Proteomic and Biochemical Methods to Study the Cytoskeletome. Fabricating Surfaces with Distinct Geometries and Different Combinations of Cell Adhesion Proteins -- Purification of Native Arp2/3 Complex from Bovine Thymus -- Purification of Arp2/3 Complex from Saccharomyces Cerevisiae -- Measurement and Analysis of in vitro Actin Polymerization -- Use of the xCELLigence System for Real Time Analysis of changes in Cellular Motility and Adhesion in Physiological Conditions -- Measuring Chemotaxis Using Direct Visualisation Microscope Chambers -- In Vitro Microtubule Severing Assays -- VE-Cadherin Status as an Indicator of Microvascular Permeability -- High-Resolution Live-Cell Imaging and Time-Lapse Microscopy of Invadopodium Dynamics and Tracking Analysis -- Live Cell Imaging of RhoGTPase Biosensors in Tumor Cells -- Electrospun Nanofiber Scaffolds for Investigating Cell-matrix Adhesion -- Method for Measuring Single-Molecule Adhesion Forces and Attachment Lifetimes of Protein-Membrane Interactions -- VE-Cadherin Status as an Indicator of Microvascular Permeability -- High-Resolution Live-Cell Imaging and Time-Lapse Microscopy of Invadopodium Dynamics and Tracking Analysis. Live Cell Imaging of RhoGTPase Biosensors in Tumor Cells -- Electrospun Nanofiber Scaffolds for Investigating Cell-matrix Adhesion -- Method for Measuring Single-Molecule Adhesion Forces and Attachment Lifetimes of Protein-Membrane Interactions Cellular adhesion is a fundamental process that influences numerous biological activities such as morphogenesis, cell motility and division, as well as signalling. In addition, adhesion is a process important not only in normal physiology and development, but also in disease states such as tumourigenesis, cardiovascular disease, inflammation and infection. There are a plethora of proteins involved in adhesion-related events with a huge diversity in function. As a result, a wide variety of techniques exist to study adhesion related proteins and processes. In Adhesion Protein Protocols, Third Edition, chapters cover techniques to gain insight into the complex and incompletely understood processes that are involved in cellular adhesion. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Adhesion Protein Protocols, Third Edition will be useful for both those new to the field of adhesion protein research as well as the more experienced scientist Coutts, Amanda S. editor SpringerLink eBooks (Servicio en línea) Springer Protocols (Servicio en línea) Methods in Molecular Biology, Methods and Protocols 1064-3745 1046