Descripción del título
A "Dual-Addition" Calcium F...
In this work, a high-throughput, intracellular calcium fluorescence assay for 384-well plates to screen small molecule libraries on recombinant G protein-coupled receptors (GPCRs) is described. The target, the kinin receptor from the cattle fever tick, Rhipicephalus microplus, is expressed in CHO-K1 cells. This assay identifies agonists and antagonists using the same cells in one "dual-addition" assay.\r\n
Material Proyectable
material_proyectable Rebiun34938620 https://catalogo.rebiun.org/rebiun/record/Rebiun34938620 m o c vz czazuu cr cnannnuuuuu 230725s2022 mau009 o vleng d 64505 JoVE 64505 MyJoVE Corporation MAJOV eng rda pn MAJOV eng eng A "Dual-Addition" Calcium Fluorescence Assay for the High-Throughput Screening of Recombinant G Protein-Coupled Receptors Cambridge JoVE 2022 Cambridge Cambridge JoVE 1 online resource (1 video file (8 min., 46 sec.)) sound, color 1 online resource (1 video file (8 min., 46 sec.)) 000846 Two-dimensional Moving Image tdi rdacontent computer c rdamedia online resource cr rdacarrier digital rdatr video file rdaft Biochemistry 1940-087X Presented by MyJoVE Corporation In this work, a high-throughput, intracellular calcium fluorescence assay for 384-well plates to screen small molecule libraries on recombinant G protein-coupled receptors (GPCRs) is described. The target, the kinin receptor from the cattle fever tick, Rhipicephalus microplus, is expressed in CHO-K1 cells. This assay identifies agonists and antagonists using the same cells in one "dual-addition" assay.\r\n Presented in English; subtitles in English Instructional and Educational Work MyJoVE Corporation production company publisher Biochemistry. 1940-087X