Descripción del título
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A protocol for fluorescent, flow cytometric quantification of senescent cancer cells induced by chemotherapy drugs in cell culture or in murine tumor models is presented. Optional procedures include co-immunostaining, sample fixation to facilitate large batch or time point analysis, and the enrichment of viable senescent cells by flow cytometric sorting.\r\n
Material Proyectable
material_proyectable Rebiun35566621 https://catalogo.rebiun.org/rebiun/record/Rebiun35566621 m o c vz czazuu cr cnannnuuuuu 230726s2022 mau015 o vleng d 64176 JoVE 64176 MyJoVE Corporation MAJOV eng rda pn MAJOV eng eng (S <> Cambridge JoVE 2022 Cambridge Cambridge JoVE 1 online resource (1 video file (14 min., 1 sec.)) sound, color 1 online resource (1 video file (14 min., 1 sec.)) 001401 Two-dimensional Moving Image tdi rdacontent computer c rdamedia online resource cr rdacarrier digital rdatr video file rdaft Cancer Research 1940-087X Non-Latin script record Presented by MyJoVE Corporation A protocol for fluorescent, flow cytometric quantification of senescent cancer cells induced by chemotherapy drugs in cell culture or in murine tumor models is presented. Optional procedures include co-immunostaining, sample fixation to facilitate large batch or time point analysis, and the enrichment of viable senescent cells by flow cytometric sorting.\r\n Presented in English; subtitles in English Instructional and Educational Work MyJoVE Corporation production company publisher Cancer Research. 1940-087X