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Analytical parasitology / M...
Parasites are of global significance in terms of human an animal health and research is constantly targeted at controlling such infections. In order for this to be effective, detailed analyses of the biology of each species, particularly at the molecular level, must be carried out to promote the development of new therapeutic or diagnostic approaches. This laboratory manual, with detailed background information and practical protocols, will be a useful guide for researchers engaged in many areas of parasitology. Most techniques described can be applied to both helminthic and protozoan parasites, although protocols relevant to individual species are also included. It is largely recommended for postgraduate and postdoctoral scientists and provides procedures for some basic techniques in immunological, microscopical, and molecular analyses along with more specialized schemes to give a multidisciplinary approach to experimental parasitology. Parasite infections are very widespread, nevertheless effective therapeuticals are not yet available. The analysis of the life cycle and the parasite host interactions at the molecular level may help in the search of the "Achilles heel" of a parasite and thus promote the development of new therapeutic approaches. Parasite molecules such as surface antigens, excretory proteins or metabolic enzymes may serve as targets for new diagnostics tests, chemo- or immunotherapeutics or even as candidate vaccine
Monografía
monografia Rebiun22059493 https://catalogo.rebiun.org/rebiun/record/Rebiun22059493 m o d cr bn||||||abp cr bn||||||ada 101117s1997 gw a ob 001 0 eng d 645937664 828770835 934989043 936313185 1001493289 1012461861 1058173881 9783642603457 electronic bk.) 3642603459 electronic bk.) 3540589198 alk. paper) 9783540589198 alk. paper) 9783642478109 3642478107 10.1007/978-3-642-60345-7 doi AU@ 000051693911 NZ1 14999952 NZ1 15300995 AU@ 000057642668 OCLCE eng pn OCLCE OCLCQ GW5XE VT2 OCLCO I9W OCLCQ OCLCF OCLCQ OCL OCLCQ EBLCP OCLCQ YDX UAB OCLCO COO OCLCA OCLCQ AU@ OCLCA dlr PSF bicssc SCI049000 bisacsh 616.9/6 20 44.43 bcl WI 3640 rvk XD 8811 rvk Analytical parasitology Michael T. Rogan (ed.). Berlin New York Springer ©1997 Berlin New York Berlin New York Springer 1 online resource (xvii, 364 pages) illustrations 1 online resource (xvii, 364 pages) Text txt rdacontent computer c rdamedia online resource cr rdacarrier text file PDF rda Springer lab manual Includes bibliographical references and index 1 Biochemical Pathways in Parasites -- 1.1 Introduction -- 1.2 Preparation of Enzyme Fractions -- 1.3 Enzyme Assays -- 1.4 Metabolite Measurements -- 1.5 Use of Inhibitors -- References -- 2 Electrophoresis of Parasite Proteins -- 2.1 Introduction -- 2.2 Lysis of Parasites and Extraction of Proteins -- 2.3 SDS-PAGE -- 2.4 Modifications of Standard Method -- 2.5 Applications of SDS-PAGE to the Characterisation of Individual Polypeptides -- References -- 3 Molecular Techniques in Analytical Parasitology -- 3.1Introduction -- 3.2 Isolation of Parasite Nucleic Acid -- 3.3 Gel Electrophoresis and Blotting of Nucleic Acid -- 3.4 Labelling and Hybridisation of DNA Probes -- References -- 4 The Production and Analysis of Helminth Excretory-Secretory (ES) Products -- 4.1 Introduction -- 4.2 Collection of Excretory-Secretory (ES) Products -- 4.3 Acetylcholinesterase -- 4.4 Proteolytic Enzymes -- 4.5 Anticoagulants, Blood Coagulation Assays -- 4.6 Potential Immunomodulators -- 4.7 Concluding Remarks -- References -- 5 Parasite Proteinases -- 5.1 Introduction -- 5.2 Sample Preparation -- 5.3 Substrates and Proteinase Assays -- 5.4 Proteinase Inhibitors -- 5.5 Electrophoretic Methods -- 5.6 Purification Methods -- 5.7 Endogenous Inhibitors -- 5.8 Final Comments -- References -- 6 Neurobiology of Helminth Parasites -- 6.1 Introduction -- 6.2 Immunocytochemical Localisation of Neuroactive Substances in Helminth Parasites -- 6.3 Characterisation and Purification of Neuropeptides -- 6.4 Physiological Action of Transmitters and Drug Assessment In Vitro -- References -- 7 Electron Microscopy in Parasitology -- 7.1 Introduction -- 7.2 Preparation for Transmission Electron Microscopy (TEM) -- 7.3 Cytochemistry and Staining Protocol in TEM -- 7.4 Preparation for Scanning Electron Microscopy (SEM) -- 7.5 Immunogold-Labelling Techniques -- References -- 8 Studies of the Surface Properties, Lipophilic Proteins and Metabolism of Parasites by the Use of Fluorescent and Caged Compounds -- 8.1 Introduction -- 8.2 Incorporation of Fluorescent Compounds into Membranes -- 8.3 Using Fluorescent Techniques to Measure Membrane Fluidity -- 8.4 Distribution of Actin -- 8.5 Assessment of Parasite Membrane Damage -- 8.6 Localisation of Probes in Organelles -- 8.7 Measurement of pH at the Surface of Parasites and Intracellular pH -- 8.8 The Use of Caged Compounds -- 8.9 Use of Fluorescent Probes in the Analysis of Parasite Proteins -- References -- 9 Detection and Identification of Parasite Surface Carbohydrates by Lectins -- 9.1 Introduction -- 9.2 Lectin-Mediated Trypanosomatid Parasite Agglutination -- 9.3 Fluorescein-Isothiocyanate (FITC)-Conjugated Lectins -- 9.4 Peroxidase-Labelled Lectins -- 9.5 Biotinylated Lectins -- 9.6 Fixatives and Enzymatic Treatments -- 9.7 Histological Sections -- 9.8 Controls -- References -- 10 Immunological Analysis of Parasite Molecules -- 10.1 Introduction -- 10.2 Parasite Extracts -- 10.3 Production of Antibodies to Parasite Molecules -- 10.4 Enzyme Linked Immunosorbent Assays (ELISA) -- 10.5 Immunoblotting -- 10.6 Affinity Chromatography -- References Use copy. Restrictions unspecified star. MiAaHDL Parasites are of global significance in terms of human an animal health and research is constantly targeted at controlling such infections. In order for this to be effective, detailed analyses of the biology of each species, particularly at the molecular level, must be carried out to promote the development of new therapeutic or diagnostic approaches. This laboratory manual, with detailed background information and practical protocols, will be a useful guide for researchers engaged in many areas of parasitology. Most techniques described can be applied to both helminthic and protozoan parasites, although protocols relevant to individual species are also included. It is largely recommended for postgraduate and postdoctoral scientists and provides procedures for some basic techniques in immunological, microscopical, and molecular analyses along with more specialized schemes to give a multidisciplinary approach to experimental parasitology. Parasite infections are very widespread, nevertheless effective therapeuticals are not yet available. The analysis of the life cycle and the parasite host interactions at the molecular level may help in the search of the "Achilles heel" of a parasite and thus promote the development of new therapeutic approaches. Parasite molecules such as surface antigens, excretory proteins or metabolic enzymes may serve as targets for new diagnostics tests, chemo- or immunotherapeutics or even as candidate vaccine Electronic reproduction. [S.l.] HathiTrust Digital Library 2010. MiAaHDL Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL English digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL Diagnostic parasitology- Laboratory manuals Parasitology- methods Diagnostic parasitology. Parasitología Parasitologia (tecnicas) Labormedizin. Parasitologie. Laboratory Manual Electronic books Laboratory manuals. Rogan, Michael T. 1960-) Print version Analytical parasitology. Berlin ; New York : Springer, ©1997 (DLC) 96033385 (OCoLC)35198679 Springer lab manual