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Apoptosis : a practical app...
This text is designed to provide conceptual outlines and detailed procedures for basic and advanced studies of cell death by apoptosis. Chapters on the recognition of apoptosis as distinguished from necrosis and nonspecific cell DNA damage, are followed by a systematic examination of the established and the principal novel methodologies utilized by some leading laboratories conducting research on apoptosis. The organization is on the lines of signalling for apoptosis, the apoptotic cascade, and the execution of apoptosis. A wide variety of procedures are provided which will enable the reader to participate in cutting-edge research
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monografia Rebiun39068509 https://catalogo.rebiun.org/rebiun/record/Rebiun39068509 m o d cr cnu---unuuu 031229s1999 enka ob 001 0 eng d 99033468 GB99W3154 bnb 666954930 666989868 814378626 824546005 1076256045 1162022836 1241750337 1409186090 0585483582 electronic bk.) 9780585483580 electronic bk.) 1280374721 9781280374722 9780191566004 0191566004 9786610374724 6610374724 0199637857 pbk.) 9780199637850 pbk.) 0199637849 hbk.) 9780199637843 hbk.) AU@ 000051574456 N$T eng pn N$T OCLCQ YDXCP OCLCQ IDEBK OCLCQ OCLCF NLGGC OCLCO OCLCQ OCL OCLCQ INTCL TOA AGLDB OCLCQ VNS OCLCQ VTS EBLCP GDC OCLCO STF OCLCQ UKSSU OCLCO VLY OCLCA OCLCO OCLCQ OCLCO OCLCL INARC OCLCA OCLCL OCLCQ (S X300 SCI 056000 bisacsh MFGC bicssc 616-091.8 571.9/36 22 https://id.oclc.org/worldcat/ddc/E4cBXXRt49mwFybTbqJ6xrhwfG 44.47 bcl XG 2111 rvk Apoptosis a practical approach edited by George P. Studzinski Oxford New York Oxford University Press 1999 Oxford New York Oxford New York Oxford University Press 1 online resource (xxii, 244 pages) illustrations (some color) 1 online resource (xxii, 244 pages) Text txt rdacontent computer c rdamedia online resource cr rdacarrier The practical approach series 216 Includes bibliographical references and index Intro; Contents; List of Contributors; Abbreviations; 1. Overview of apoptosis; 1. General introduction and overview of contents; 2. Historical perspective; 3. Distinction of apoptosis from other forms of cell death; 4. Apoptotic cascades; 5. Time course of apoptotic cascades; 6. Selection of methods; Procedure for determination of increased mitochondrial to nuclear DNA ratio, for the detection and quantitation of apoptosis; 7. Pitfalls; References; 2. Morphological recognition of apoptotic cells; 1. Introduction; Key morphological features of apoptotic cells 2. Light and fluorescent microscopy techniques for the assessment of apoptosisPreparation of cell or tissue samples; Nuclear counterstains; 3. Electron microscopic techniques; 4. Quantitation of apoptotic events; Methods; Problems in scoring apoptotic events; 5. In situ detection of DNA strand breaks; 6. Other techniques; 7. Conclusions; References; 3. Assessment of DNA damage in apoptosis; 1. Introduction; 2. Types of DNA fragmentation; 3. Measurement of high molecular weight DNA fragmentation by pulse-field gel electrophoresis; Pulse-field gel electrophoresis procedures Strategies for electrophoretic separations4. Detection of internucleosomal DNA fragmentation by standard agarose gel electrophoresis; 5. Filter elution assay to measure apoptotic DNA fragmentation; Equipment; Filters and solutions; DNA labelling and preparation of experimental cell cultures; Protocols; Counting samples and computations; References; 4. Analysis of cell death by flow and laser-scanning cytometry; 1. Introduction; 2. Preparation of cells for analysis by LSC. Detection of apoptotic cells based on changes in nuclear chromatin Fas-based cytotoxicityPerforin/granzyme-based cytotoxicity; 2. Standard methods for the evaluation of cell-mediated cytotoxicity; Chromium release assay; [[sup(125)]I]Iododeoxyuridine ([sup(125)]IUdR) release assay; The JAM test; BLT-esterase release assay; Estimation of target cell nuclear fragmentation using fluorescent dyes; Activation-induced cell death (AICD); 3. Separate studies of Fas- and perforin/granzyme-based cytotoxicity; Fas-based cytotoxicity in the absence of perforin/granzyme contribution; Perforin/granzyme-based cytotoxicity in the absence of FasL contribution This text is designed to provide conceptual outlines and detailed procedures for basic and advanced studies of cell death by apoptosis. Chapters on the recognition of apoptosis as distinguished from necrosis and nonspecific cell DNA damage, are followed by a systematic examination of the established and the principal novel methodologies utilized by some leading laboratories conducting research on apoptosis. The organization is on the lines of signalling for apoptosis, the apoptotic cascade, and the execution of apoptosis. A wide variety of procedures are provided which will enable the reader to participate in cutting-edge research English Apoptosis- Laboratory manuals Apoptosis Apoptosis Biology Health & Biological Sciences Cytology Apoptose- Manuels de laboratoire Apoptose SCIENCE- Life Sciences- Anatomy & Physiology. Apoptosis. Apoptose. Labortechnik. Apoptosis. Celdood. Apoptose. Laboratory Manual Laboratory manuals. Laboratory manuals. Manuels de laboratoire. Studzinski, George P. Print version Apoptosis. Oxford ; New York : Oxford University Press, 1999 0199637857 0199637849 (DLC) 99033468 (OCoLC)41580448 Practical approach series 216