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Basic cloning procedures / ...
This manual introduces the reader to bacic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis. Each protocol includes short background information, a detailed description of the necessary materials, step-by-step procedures, a troubleshooting guide and useful practical hints
Monografía
monografia Rebiun32990968 https://catalogo.rebiun.org/rebiun/record/Rebiun32990968 m o d cr bn||||||abp cr bn||||||ada 101107s1998 gw a ob 001 0 eng d 645909697 840294852 968665970 1001479863 1086546667 1112431695 9783642719653 electronic bk.) 3642719651 electronic bk.) 9783642489778 print) 364248977X print) 3540635394 wire-o-bind ; alk. paper) 9783540635390 wire-o-bind ; alk. paper) 10.1007/978-3-642-71965-3 doi AU@ 000051702624 NZ1 15001866 NZ1 15300877 OCLCE eng pn OCLCE OCLCQ I9W OCLCO GW5XE OCLCQ OCLCF OCLCQ UA@ COO OCL OCLCQ EBLCP OCLCQ YDX UAB OCLCO OCLCA OCLCQ OCLCA AU@ OCLCO OCLCA OCLCQ OCLCO LEAUB OCLCQ OCLCA OCLCQ OCLCO OCLCQ dlr PSB bicssc SCI007000 bisacsh PSB thema 572.8/645 21 42.20 bcl 42.03 bcl WC 4460 rvk WG 3450 rvk BIO 180f stub Basic cloning procedures Valdis Berzins (ed.). Berlin New York Springer 1998 Berlin New York Berlin New York Springer 1 online resource (169 pages) illustrations 1 online resource (169 pages) Text txt rdacontent computer c rdamedia online resource cr rdacarrier text file PDF Springer lab manual Includes bibliographical references and index 1 cDNA Synthesis and Cloning -- 2 DNA Sequencing -- 3 RNA In Vitro Synthesis by Phage T7 DNA-Dependent RNA Polymerase -- 4 PCR-Based Site-Specific Mutagenesis -- 5 Analysis of Specific Protein-DNA Interactions -- 6 Gel Electrophoresis, Transfer and Hybridization of Nucleic Acids -- 7 Immunological Methods for Analysis of Recombinant Proteins -- 8 Protein Synthesis in Cell-Free Systems Use copy. Restrictions unspecified star. MiAaHDL This manual introduces the reader to bacic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis. Each protocol includes short background information, a detailed description of the necessary materials, step-by-step procedures, a troubleshooting guide and useful practical hints Electronic reproduction. [Place of publication not identified] HathiTrust Digital Library 2010. MiAaHDL Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL Molecular cloning- Laboratory manuals Molecular cloning Cloning, Molecular Clonage moléculaire- Manuels de laboratoire Clonage moléculaire Molecular cloning. Genklonierung. Molekularbiologie. Kloneren. Wetenschappelijke technieken. Laboratory Manual Laboratory manuals. Laboratory manuals. Manuels de laboratoire. Berzins, Valdis 1943-) Print version Basic cloning procedures. Berlin ; New York : Springer, 1998 (DLC) 97043014 (OCoLC)37813544 Springer lab manual