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Cellular and molecular meth...
Analysis of the neural tissue presents unique and peculiar technical problems encountered in everyday bench work. Numerous books dealing with cellular and molecular protocols for general use in cell biology are available, but few are specifically devoted exclusively to neurobiology. Moreover, the "cross-talk" between researchers with different backgrounds, i.e. histologists, cell and molecular biologists and physiologists, is still quite difficult, and very often one remains somehow "confined" to his or her own specific field of expertise never daring to explore "mysterious" lands unless having the support of a big laboratory beyond. The general idea beyond this project was to put together the contributions from a number of well- known neuroscientists to produce a book that offers a survey of the most updated techniques for the study of nerve cells. After a long time spent doing research in the neuroscience field, and having acquired a good technical background in certain specific fields of neurobiology we have realized how difficult is to be able to step into a different technology. This book endeavors to assist in that goal
Monografía
monografia Rebiun25259998 https://catalogo.rebiun.org/rebiun/record/Rebiun25259998 m o d cr cn||||||||| 011012s2002 nyua ob 001 0 eng d 53337505 55050766 133157971 229457239 456042923 465031620 551393000 559289010 647289513 722205120 793528225 808036508 814404178 888728399 961615368 961643997 962656292 962681302 1044207281 1056332111 1058952202 0387224602 electronic bk.) 9780387224602 electronic bk.) 0585472726 electronic bk.) 9780585472720 electronic bk.) 1280009527 9781280009525 661000952X 9786610009527 0387953868 9780387953861 10.1007/b98863 doi AU@ 000053244839 AU@ 000054931603 DEBSZ 430279973 NZ1 11922917 NZ1 15042963 REDDC eng pn REDDC OCLCQ N$T UAB YNG DKDLA OKU MERUC CCO E7B OCLCQ YDXCP OCLCO OCLCQ GW5XE OCLCF OCLCQ QE2 IDEBK OCLCQ NLGGC OCLCQ COO EBLCP AZU DEBSZ OCLCQ OCL OCLCQ AZK MOR OCLCQ OCLCO OCLCA STF OCLCO OCLCQ CEF NRAMU INT OCLCQ AU@ GDC OCLCQ OCLCO CNTRU M8D OCLCQ OCLCA OCLCQ OCLCA SOC 002020 bisacsh PSAN bicssc 573.8/48 21 Cellular and molecular methods in neuroscience research editors, Adalberto Merighi, Giorgio Carmignoto New York Springer 2002 New York New York Springer 1 online resource (1 volume (various pagings)) illustrations (some color) 1 online resource (1 volume (various pagings)) Text txt rdacontent computer c rdamedia online resource cr rdacarrier data file rda Includes bibliographical references and index Analyses of Intracellular Signal Transduction Pathways in CNS Cells -- Confocal and Electron Microscopic Trakcking of Internalized Neuropeptide/Receptor Complexes -- Transfection Methods for Neurons in Primary Culture -- Polyethylenimine: a Versatile Cationic Polymer for Plasmid-based Gene Delivery in the CNS -- Trasfection of GABAA Receptor with GFP-Tagged Subunits in Neurons and HEK293 Cells -- Neuronal Transfection Using Particle-Mediated Gene Transfer -- Analysis of Gene Expression in Genetically Labeled Single Cells -- Immunocytochemistry and In Situ Hybridization: Interest of their Combinations for Cytofunctional Approaches of Central and Peripheral Neurons -- In Situ Reverse Transcriptase PCR for Detection of mRNA in the CNS -- Immunocytochemical Labeling Methods and Related Techniques for Ultrastructural Analysis of Neuronal Connectivity -- Combined electrophysiological and morphological analysis of CNS neurons -- Tract -Tracing Methods at the Light Microscopy Level -- Tract-tracing Methods at the Ultrastructural Level -- In vivo Analysis of Cell Proliferation and Apoptosis in the CNS -- Confocal Imaging of Nerve Cells and their Connections -- Confocal Imaging of Calcium Signaling in Cells from Analysis of the neural tissue presents unique and peculiar technical problems encountered in everyday bench work. Numerous books dealing with cellular and molecular protocols for general use in cell biology are available, but few are specifically devoted exclusively to neurobiology. Moreover, the "cross-talk" between researchers with different backgrounds, i.e. histologists, cell and molecular biologists and physiologists, is still quite difficult, and very often one remains somehow "confined" to his or her own specific field of expertise never daring to explore "mysterious" lands unless having the support of a big laboratory beyond. The general idea beyond this project was to put together the contributions from a number of well- known neuroscientists to produce a book that offers a survey of the most updated techniques for the study of nerve cells. After a long time spent doing research in the neuroscience field, and having acquired a good technical background in certain specific fields of neurobiology we have realized how difficult is to be able to step into a different technology. This book endeavors to assist in that goal English Molecular neurobiology- Laboratory manuals Neurons- Laboratory manuals Neurobiology Neurons- physiology SOCIAL SCIENCE- Anthropology- Physical. Molecular neurobiology. Neurons. Laboratory Manual Electronic books Laboratory manuals. Merighi, Adalberto Carmignoto, Giorgio Print version Cellular and molecular methods in neuroscience research. New York : Springer, 2002 (DLC) 2001054917