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Chromosome microdissection ...
Monografía
monografia Rebiun26047136 https://catalogo.rebiun.org/rebiun/record/Rebiun26047136 m o d cr bn||||||abp cr bn||||||ada 101117s1993 caua ob 001 0 eng d 643888121 842859981 974618009 974669495 987731272 988556507 1299193684 ebk) 9781299193680 ebk) 9780123133205 0123133203 DEBBG BV042302869 AU@ 000061146467 UPVA 997186036303706 450618 MIL OCLCE eng pn OCLCE OCLCQ OPELS OCLCQ E7B OCLCF OCLCQ IDEBK UIU OCLCQ OCLCO OCLCQ OCLCA LEAUB OCLCO LUN dlr 575.1/0724 20 BIO 180f stub BIO 220f stub BIO 750f stub WC 4400 rvk WG 3450 rvk BIO 180f. stub BIO 220f. stub BIO 750f. stub WC 4400. rvk WG 3450. rvk Chromosome microdissection and cloning a practical guide [edited by] Nabil G. Hagig and Michael V. Viola San Diego Academic Press 1993 San Diego San Diego Academic Press 1 online resource (x, 160 pages) illustrations 1 online resource (x, 160 pages) Text txt rdacontent computer c rdamedia online resource cr rdacarrier Includes bibliographical references and index Introduction to chromosome microdissection -- Chromosome organization -- Cloning DNA from chromosome fragments -- Preparation of chromosomes for microdissection -- Critical aspects of chromosome preparation -- Enrichment of metaphase spreads -- Hypotonic treatment -- Chromosome fixing and spreading -- Aging, storing, and staining of metaphase spreads -- Reagents -- Equipment -- Protocols -- Protocol 1. Preparation of chromosomes from peripheral blood T lymphocytes (whole blood microculture method) -- Protocol 2. Preparation of chromosomes from monolayer tissue culture cell lines -- Protocol 3. Preparation of chromosomes from monolayer cells grown on coverslips -- Protocol 4. Preparation of chromosomes from dipteran salivary glands -- Protocol 5. Solid staining and GTG banding of metaphase chromosomes -- Methods of chromosome microdissection -- Methods -- Video microscope method -- Oil chamber method -- Laser microdissection method -- Summary of chromosome microdissection and collection for DNA cloning -- Reagents and equipment -- Protocol (cont) Molecular cloning of microdissected chromosal DNA -- Cloning of DNA from microdissected chromosomal DNA fragments -- Method 1. Direct cloning of DNA from microdissected chromosomal fragments -- Direct cloning into [gamma] phage -- Method 2. Ligation of microdissected chromosomal DNA with plasmid vector or linker-adaptor and PCR amplification -- Protocol 2.1. Ligation of microdissected DNA with plasmid vector, PCR amplification, and cloning -- Protocol 2.2. Ligation of microdissected DNA with linker-adaptor, PCR amplification, and cloning -- Method 3. PCR amplification of microdissected chromosomal DNA fragments followed by probing a complete recombinant library -- Protocol 3.1. Preparation of chromosomal DNA for amplification -- Protocol 3.2. PCR amplification of microdissected chromosomal DNA using "universal" primers -- Protocol 3.3. PCR amplification using human Alu sequence-based primers -- Analysis of recombinant clones derived from microdissected chromosomal DNA -- Determination of DNA insert size range -- Determination of the percentage of recombinant clones containing repeat and unique sequences (cont) Protocol 4.1. Assay for repeat sequences -- Protocol 4.2. Assay for unique sequences -- Calculation of the percentage of total microdissected DNA cloned -- Determination of potential structural gene sequences -- Localization of recombinant clones using in situ hybridization -- Protocol 5.1. DNA probe labeling for fluorescence in situ hybridization -- Protocol 5.2. Fluorescent in situ hybridization to metaphase chromosome spreads -- Applications of chromosome microdissection -- Direct analysis of the PCR product of microdissected chromosome fragments -- Gene mapping -- Mapping sites of chromosome rearrangement and deletions -- Determination of coupling phase -- Recombinant DNA libraries generated from microdissected chromosome fragments -- Genetic analysis of specialized chromosome structures -- Applications in genomic sequencing projects -- Characterization of disease-related genetic loci -- Study of chromosome abnormalities in cancer cells -- Gene transfer using chromosome fragments Use copy. Restrictions unspecified star. MiAaHDL Electronic reproduction. [S.l.] HathiTrust Digital Library 2010. MiAaHDL Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL Molecular cloning- Technique Microdissection Chromosomes Chromosomes- ultrastructure- Laboratory Manuals Cloning, Molecular- methods- Laboratory Manuals Dissection- methods- Laboratory Manuals Chromosomes. Microdissection. Molecular cloning- Technique. Chromosom. Mikromanipulator. Chromosomes Analysis Laboratory Manual Electronic books Hagag, Nabil G. Viola, Michael V. Print version Chromosome microdissection and cloning. San Diego : Academic Press, 1993 (DLC) 93007286 (OCoLC)27816787